VOLUME 14 NUMBER 1 (January to July 2021)

PSL%202021 vol14-no01-p12-28-Mikita%20and%20Padlan

Philipp. Sci. Lett. 2021 14 (1) 101-107
available online: April 29, 2021

*Corresponding author
Email Address: eduardo.padlan@gmail.com
Date received: March 2, 2021
Date revised: April 20, 2021
Date accepted: April 23, 2021

ARTICLE

Design of a possible subunit vaccine against SARS-CoV-2

Cecilia P. Mikita1 and Eduardo A. Padlan*,2
1Walter Reed National Military Medical Center,
      Department of Medicine,
      Allergy/Immunology/Immunizations Service,
      4954 North Palmer Road, Bethesda, MD 20889-5600, USA
24006 Simms Drive, Kensington, MD 20895-1336, USA
A possible subunit vaccine that focuses the immune response to the receptor-binding site of the spike glycoprotein of SARS-CoV-2 is designed. Sequence and three-dimensional structural data are used to map the epitopes of neutralizing antibodies on a suitable spike protein for use as a subunit vaccine. The chemical reactivity of the residues in the spike protein is reduced by judicious amino acid replacements, except for the residues in the epitopes. This results in a molecule that would elicit an immune response directed at the known epitopes. The proposed subunit vaccine is expected to work regardless of the existence of mutations in one or more, but not all, of the epitopes, as well as elsewhere in the spike protein. The amino acid sequence of the proposed vaccine may serve as the starting point for even more efficacious vaccines if more neutralizing antibody epitopes are included.

© 2020 Philippine Science Letters